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人抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯(lián)免疫吸附檢測試劑盒

ELK9590

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit
Reactivity:	Human
Alternative Names:	ACP5;TRAP5b;SPENCDI
Assay Type:	Sandwich
Sensitivity:	0.45 ng/mL
Standard:	50 ng/mL
Detection Range:	0.78-50 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal Transduction;Signal Transduction;Metabolism
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

Frontiers in Pharmacology

Yigu decoction regulates plasma miRNA in postmenopausal osteoporosis patients: a randomized controlled trial

日期：2025-01-17 IF：4.4

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
50.00	2.204	2.117
25.00	1.543	1.456
12.50	1.173	1.086
6.25	0.907	0.820
3.13	0.525	0.438
1.57	0.369	0.282
0.79	0.267	0.180
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TRACP-5b and the recovery rates were calculated by comparing the measured value to the expected amount of TRACP-5b in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	96-107%	102%
Heparin plasma(n=5)	80-93%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRACP-5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.