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人鋅指蛋白746(ZNF746)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK8990

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Human ZNF746(Zinc finger protein 746) ELISA Kit
Reactivity:	Human
Alternative Names:	FLJ31413; zinc finger protein 746; ZNF746
Assay Type:	Sandwich
Sensitivity:	6.5 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Neuro science
Uniprot ID:	Q6NUN9
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ZNF746. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ZNF746. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ZNF746, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ZNF746 in the samples is then determined by comparing the OD of the samples to the standard curve.

IBRO Neuroscience Reports

Genetic variants of ZNF746 and the level of plasma Parkin, PINK1, and ZNF746 proteins in patients with Parkinson's disease

日期：2025-03-17 IF：2

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.258	2.172
500.00	1.699	1.613
250.00	1.147	1.061
125.00	0.834	0.748
62.50	0.597	0.511
31.25	0.372	0.286
15.63	0.216	0.130
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ZNF746 and the recovery rates were calculated by comparing the measured value to the expected amount of ZNF746 in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	79-93%	86%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ZNF746 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.