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小鼠沉默調(diào)節(jié)蛋白1(SIRT1)酶聯(lián)免疫吸附檢測試劑盒

ELK6397

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Mouse SIRT1(Sirtuin 1) ELISA Kit
Reactivity:	Mouse
Alternative Names:	SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment
Assay Type:	Sandwich
Sensitivity:	0.122 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;
Uniprot ID:	Q923E4
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

PHYTOMEDICINE

Xiyangshen Sanqi Danshen granules attenuated D-gal-induced C57BL/6J mouse aging through the AMPK/SIRT1 signaling pathway

日期：2025-01-17 IF：6.7
BIOLOGICAL TRACE ELEMENT RESEARCH

Anti-Obesity Effects of Calcium Fructoborate by Inhibiting Adipogenesis and Increasing SIRT’s Expression in 3T3-L1 Cells

日期：2025-01-17 IF：3.4

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.056	1.973
10.00	1.657	1.574
5.00	1.186	1.103
2.50	0.852	0.769
1.25	0.559	0.476
0.63	0.361	0.278
0.32	0.302	0.219
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant SIRT1 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT1 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	80-92%	86%
Heparin plasma(n=5)	92-107%	99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.